Anatomy; Cell Culture Techniques; Histology, Comparative; Microscopy; Molecular Biology
The neuromuscular junction connects nervous system and muscle tissue. Muscle specific kinase (MuSK) is a receptor tyrosine kinase that is crucial for its formation. I aim to characterize a specific serine residue in MuSK that relates to its activation. I will identify the kinase that phosphorylates this serine, and analyse the kinase domains of wildtype- and phosphomimetic mutant MuSK in a structure-function approach. A transgenic mouse model will provide understanding of the effect of serine phosphorylation in vivo. These data will provide insight in the mechanism of MuSK activation and potential rescue mechanisms for the development of the neuromuscular junction.
Techniques, methods & infrastructure
Cell culture, Western Blot, Molecular Cloning, Fast Protein Liquid Chormatography, Histology and Immunocytochemistry, Fluorescence- and confocal laser scanning microscopy